Antibodies - A LABORATORY MANUAL - download pdf or read online

By Ed Harlow and David Lane

ISBN-10: 0879693142

ISBN-13: 9780879693145

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1980. Immunoglobulin gene rearrangement in immature B cells. Science 209: 1366- 1369. , G. S. H. Gell. 1965. Cellular localiza- 22 CHAPTER 2 tion of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J. Exp. Med. 122:853-875. E. Kelley, C. F. Kearney. 1981. Organization and expression of immunoglobulin genes in fetal liver hybridomas. Proc. Natl. Acad. Sci. 78:247-251. L. Brinster, and U. Storb. 1984. Allelic exclusion and control of endogenous immunoglobulin gene rearrangement in K transgenic mice.

Sci. 78: 7674-7678. , M. Davis, E. Sinn, P. Patten, and L. Hood. 1981. Antibody diversity: Somatic hypermutation of rearranged VH genes. Cell 27:573-581. , J. Hochtl, H. G. Zachau. 1981. , coding sequences suggest a localized mutation mechanism. Nature 291:668-670. Seising, E. and U. Storb. 1981. Somatic mutation of immunoglobulin lightchain variable-region genes. Cell 25: 47- 58. Y. L. Gefter. 1983. The genetic basis of antibody production: A single heavy chain variable region gene encodes all molecules bearing the dominant anti-arsonate idiotype in the strain A mouse.

The second signal is mediated by the binding of a soluble polypeptide factor known as interleukin 1 (IL-l). IL-l is produced by the APC as well as by many other cell types. The production of IL-l is nonspecific, but within the helper T -cell population, only T cells that are bound to an APC will respond. The next step in the proliferation and differentiation of helper T cells involves a second well-characterized polypeptide growth factor, interleukin 2 (IL-2). IL-2 is a 15,000-dalton glycoprotein that is secreted by T cells in response to the combined stimulation of antigen binding and IL-l.

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Antibodies - A LABORATORY MANUAL by Ed Harlow and David Lane


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