By Jac A. Nickoloff
Harvard college, Boston, Massachusetts. guide of protocols within the electroporation of proteins and DNA into fish, insect, and mammalian cells. For researchers. Plastic comb spiral binding. sixty five individuals, 28 U.S.
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Extra info for Animal Cell Electroporation and Electrofusion Protocols (Methods in Molecular Biology)
Eur. J Biochem. 209,431-436. 4. Morgan, W. F. and Wmegar, R. A. (1990) The use of restriction endonucleases to study the mechamsms of chromosome damage, in Chromosomal Aberrations’ Basic and Applied Aspects (Obe, G. and Natarajan, A. ), Springer-Verlag, Berlin, pp. 70-78 5 Morgan, W. , Chung, H. , Ortiz, T , Phillips, J. , and Winegar, R. A. (1990) The cytogenetrc effects of restriction endonucleases following their introduction into cells by electroporation, in Mutation and the Environment, Part B: Metabolism, Testing Methods, and Chromosomes (Mendelsohn, M.
A. (1990) The cytogenetrc effects of restriction endonucleases following their introduction into cells by electroporation, in Mutation and the Environment, Part B: Metabolism, Testing Methods, and Chromosomes (Mendelsohn, M. L. and Albertini, R. ), Wiley-Liss, New York, pp. 355-361. 6 Shigekawa, K. and Dower, W. J.
Remove the trypsin/EDTA by pouring off the supernatant after centrtfugation at approx 3OOg. 3. Wash the cell pellet, either in serum-free medium or in HeBS, at 4OC. 4. Recentrtfuge the cells and resuspend them m HeBS at 4OC to 2 x lo6 cells/ml. 5. Put 800 uL of the cell suspension in a disposable electroporation cuvet, and add restriction enzyme. Homogenize by gently inverting the cuvet ten times. 6. 4-cm electrode gap), capacitance 1600 uF, and the electroporator set at low resistance. 7. Carefully remove the cell suspension from the cuvet, and mix with 10 mL of medium in a tissue-culture dish.
Animal Cell Electroporation and Electrofusion Protocols (Methods in Molecular Biology) by Jac A. Nickoloff
Categories: Molecular Biology